Hehe Chris beat me to it.
# - Will work on all channels.
#Channel - Will only work in the channel #Channel.
#Channel,#mIRC,#Andy - Will only work in the following 3 channels you can specify more than 3 channels by seperating them by a comma.
As Chris said, you want it to only trigger when you're an operator otherwise you'll get an error like:
* Hooligan: you're not channel operator
So it is important to leave the @ sign there which checks if you're an operator on the channel before performing any commands.
@ is the equivalent of if ($me isop $chan) { .. }