Try this:
Code:
on *:JOIN:#AnimeOST.net: { 
  .notice $nick Hello and welcome to AnimeOST.net IRC chan. We hope you have a good time here with us and enjoy your stay. Please make sure to read the chan rules. you can do so by typing !rules in the chat window. 
  .notice $nick If you need any IRC help you can type !help and get help with registering your nick or any other IRC commands you would like to know ^_^ Have fun and enjoy your stay!
}

on *:TEXT:!help:[color:red]#AnimeOST.net[/color]:{
.notice $nick This will help you learn the IRC commands.
.notice $nick You can register your nick by typing /nickserv REGISTER <password> <email> 
.notice $nick Password being your desired password. and Email being your valid email.
}


You were missing the #channel in the TEXT event. I also made the messages silent, so you won't see them being sent. You won't be able to test the messages yourself, so someone else will have to test it for you.

-genius_at_work